In order to investigate the hypocholesteremic effect of soybean peptides, soybean protein(ISP), casein(CNP), their peptic hypdrolyzates fractionated by acidprecipitation at different pH's(SHT, SH8, SH6, SH4, CHT, CH6, CH5, CH4) and amino acid
mixtures
of the same composition as the proteins(SAA, CAA) were fed to rats and the concentration of serum cholesterol was measured. Then in vitro digestibility and molecular weight distribution of the peptides by peptic-pancreatic hydrolysis was measured
by
FPLC. The lower the in vitro digestibility of peptides is, the lower the concentration of serum cholesterol becomes(r=0.986) and the higher the ratio of macropeptides is, the lower the concentration of serum cholesterol becomes(r=-0.932) in rats.
These
results suggest that the in vitro digesthiblity of peptides has close relationship to the concentration of serum cholesterol in rats and non-digestible macropeptides or polypeptides especially more than 1 kDa, formed through digestion in gut, may
lwer
the serum cholesterol in rats
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